|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
25/09/2016 |
Actualizado : |
09/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ARRUABARRENA, A.; BENITEZ-GALEANO, M.J.; GIAMBIASI, M.; BERTALMIO, A.; COLINA, R.; HERNÁNDEZ-RODRÍGUEZ, L. |
Afiliación : |
ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; MARIO ALEJANDRO GIAMBIASI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Noroeste (CENUR Noroeste), Universidad de la República; LESTER HERNÁNDEZ-RODRÍGUEZ, Instituto de Investigaciones en Fruticultura Tropical, La Habana, Cuba. |
Título : |
Application of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Journal of Virological Methods, 2016, v.237, p. 14-17. |
DOI : |
10.1016/j.jviromet.2016.08.011 |
Idioma : |
Inglés |
Notas : |
Article history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. |
Contenido : |
ABSTRACT.
Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic
acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species.
© 2016 Elsevier B.V. All rights reserved |
Palabras claves : |
DNA; PCR; PLANT VIROID; PLANT VIRUS; PURIFICATION; RNA; RT-PCR. |
Thesagro : |
CITRUS. |
Asunto categoría : |
-- |
Marc : |
LEADER 02137naa a2200301 a 4500 001 1055729 005 2019-10-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1016/j.jviromet.2016.08.011$2DOI 100 1 $aARRUABARRENA, A. 245 $aApplication of a simple and affordable protocol for isolating plant total nucleic acids for RNA and DNA virus detection.$h[electronic resource] 260 $c2016 500 $aArticle history: Received 30 May 2016 / Received in revised form 26 July 2016 / Accepted 14 August 2016 / Available online 16 August 2016. 520 $aABSTRACT. Standard molecular methods for plant virus diagnosis require the purification of RNA or DNA extracts from a large number of samples, with sufficient concentration and quality for their use in PCR, RT-PCR, or qPCR analysis. Most methods are laborious and use either hazardous and/or costly chemicals.Apreviously published protocol for RNA isolation from several plant species yields high amounts of good quality RNADNA mixture in a simple, safe and inexpensive manner. In the present work, this method was tested to obtain RNA-DNA extracts from leaves of tomato, potato and three species of citrus, and was compared with two commercial kits. The results demonstrated that this protocol offers at least comparable nucleic acid quality, quantity and purity to those provided by commercial phenol-based or spin column systems and that are suitable to be used in PCR, RT-PCR and qPCR for virus and viroid detection. Because of its easy implementation and the use of safe and inexpensive reagents, it can be easily implemented to work in plant virus and viroid detection in different plant species. © 2016 Elsevier B.V. All rights reserved 650 $aCITRUS 653 $aDNA 653 $aPCR 653 $aPLANT VIROID 653 $aPLANT VIRUS 653 $aPURIFICATION 653 $aRNA 653 $aRT-PCR 700 1 $aBENITEZ-GALEANO, M.J. 700 1 $aGIAMBIASI, M. 700 1 $aBERTALMIO, A. 700 1 $aCOLINA, R. 700 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 773 $tJournal of Virological Methods, 2016$gv.237, p. 14-17.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
29/06/2022 |
Actualizado : |
02/12/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
FERRARI, V.; GIL, G.; HEINZEN, H.; ZOPPOLO, R.; IBÁÑEZ, F. |
Afiliación : |
VIRGINIA PAULINA FERRARI MORENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUILLERMO GIL, Laboratorio de Química de Productos Naturales, Departamento de Química Orgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay; HORACIO HEINZEN, Laboratorio de Química de Productos Naturales, Departamento de Química Orgánica, Facultad de Química, Universidad de la República, Montevideo, Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FACUNDO IBÁÑEZ SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Influence of Cultivar on Nutritional Composition and Nutraceutical Potential of Pecan Growing in Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Nutrition, 2022, volume 9, e868054. OPEN ACESS. doi: https://doi.org/10.3389/fnut.2022.868054 |
DOI : |
10.3389/fnut.2022.868054 |
Idioma : |
Inglés |
Notas : |
Article history: Received 02 February 2022; Accepted 30 May 2022; Published: 22 June 2022. -- Correspondence: Facundo Ibáñez, fibanez@inia.org.uy -- Edited by: Elena Ibañez, Institute of Food Science Research (CSIC), Spain. Reviewed by: Anabela Marisa Azul, University of Coimbra, Portugal; Lucia Panzella, University of Naples Federico II, Italy; Abraham Wall-Medrano, Universidad Autónoma de Ciudad Juárez, Mexico. LICENCE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). -- This article is part of the research topic "Insights into Food Science and Technology in Latin America" - https://www.frontiersin.org/research-topics/27424/insights-into-food-science-and-technology-in-latin-america#articles |
Contenido : |
ABSTRACT. - Composition and antioxidant properties of sixteen pecan [Carya illinoinensis (Wangenh) K. Koch] cultivars grown simultaneously in a single experimental orchard in Uruguay were evaluated to characterize their nutritional properties and nutraceutical potential. The percentage of oil, moisture, ash, minerals, and proteins were determined and also the fatty acid profile. Total phenolic compounds [18-41 mg gallic acid equivalents (GAEs)/g], condensed tannin [2-12 mg catechin equivalents (CEs)/g], and tocopherols (110-163 ?g/g) contents were estimated in nut kernels. Total phenolic compounds (32-117 mg GAE/g), condensed tannins (130-357 mg CE/g), and total anthocyanins (1-3 mg 3-glucoside cyanidin/g) were also determined for pecan shells. The antioxidant activity in shells [57.15-578.88 ?mol Trolox equivalents (TEs)/g] was 5 times higher compared with the kernels (23.15-156.60 ?mol TEs/g) measured with hydrophilic ORAC. Bioactive compounds concentrations present statistically significant genetic variability between cultivars studied (p < 0.05). The presence of phenolic compounds was related with high-antioxidant capacity in kernels and shells, and a strong correlation between content of total phenolic compounds and condensed tannins in pecan shells was found. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) show association between cultivars and the observed variables. The nutritional profile in the different cultivars showed the trends described in other countries, but this work shows some significant differences that could be attributed to the specific edaphoclimatic conditions of cultivation in Uruguay.
Copyright © 2022 Ferrari, Gil, Heinzen, Zoppolo and Ibáñez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). MenosABSTRACT. - Composition and antioxidant properties of sixteen pecan [Carya illinoinensis (Wangenh) K. Koch] cultivars grown simultaneously in a single experimental orchard in Uruguay were evaluated to characterize their nutritional properties and nutraceutical potential. The percentage of oil, moisture, ash, minerals, and proteins were determined and also the fatty acid profile. Total phenolic compounds [18-41 mg gallic acid equivalents (GAEs)/g], condensed tannin [2-12 mg catechin equivalents (CEs)/g], and tocopherols (110-163 ?g/g) contents were estimated in nut kernels. Total phenolic compounds (32-117 mg GAE/g), condensed tannins (130-357 mg CE/g), and total anthocyanins (1-3 mg 3-glucoside cyanidin/g) were also determined for pecan shells. The antioxidant activity in shells [57.15-578.88 ?mol Trolox equivalents (TEs)/g] was 5 times higher compared with the kernels (23.15-156.60 ?mol TEs/g) measured with hydrophilic ORAC. Bioactive compounds concentrations present statistically significant genetic variability between cultivars studied (p < 0.05). The presence of phenolic compounds was related with high-antioxidant capacity in kernels and shells, and a strong correlation between content of total phenolic compounds and condensed tannins in pecan shells was found. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) show association between cultivars and the observed variables. The nutritional profile in the different cultivars showed the trends descri... Presentar Todo |
Palabras claves : |
Antioxidant activity (AA); Carya illinoinensis; Fatty acids; Nuts; ORAC; PLATAFORMA AGROALIMENTOS; Polyphenols; Tannins; Tocopherols. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16899/1/fnut-09-868054.pdf
|
Marc : |
LEADER 03508naa a2200301 a 4500 001 1063359 005 2022-12-02 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fnut.2022.868054$2DOI 100 1 $aFERRARI, V. 245 $aInfluence of Cultivar on Nutritional Composition and Nutraceutical Potential of Pecan Growing in Uruguay.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 02 February 2022; Accepted 30 May 2022; Published: 22 June 2022. -- Correspondence: Facundo Ibáñez, fibanez@inia.org.uy -- Edited by: Elena Ibañez, Institute of Food Science Research (CSIC), Spain. Reviewed by: Anabela Marisa Azul, University of Coimbra, Portugal; Lucia Panzella, University of Naples Federico II, Italy; Abraham Wall-Medrano, Universidad Autónoma de Ciudad Juárez, Mexico. LICENCE: This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). -- This article is part of the research topic "Insights into Food Science and Technology in Latin America" - https://www.frontiersin.org/research-topics/27424/insights-into-food-science-and-technology-in-latin-america#articles 520 $aABSTRACT. - Composition and antioxidant properties of sixteen pecan [Carya illinoinensis (Wangenh) K. Koch] cultivars grown simultaneously in a single experimental orchard in Uruguay were evaluated to characterize their nutritional properties and nutraceutical potential. The percentage of oil, moisture, ash, minerals, and proteins were determined and also the fatty acid profile. Total phenolic compounds [18-41 mg gallic acid equivalents (GAEs)/g], condensed tannin [2-12 mg catechin equivalents (CEs)/g], and tocopherols (110-163 ?g/g) contents were estimated in nut kernels. Total phenolic compounds (32-117 mg GAE/g), condensed tannins (130-357 mg CE/g), and total anthocyanins (1-3 mg 3-glucoside cyanidin/g) were also determined for pecan shells. The antioxidant activity in shells [57.15-578.88 ?mol Trolox equivalents (TEs)/g] was 5 times higher compared with the kernels (23.15-156.60 ?mol TEs/g) measured with hydrophilic ORAC. Bioactive compounds concentrations present statistically significant genetic variability between cultivars studied (p < 0.05). The presence of phenolic compounds was related with high-antioxidant capacity in kernels and shells, and a strong correlation between content of total phenolic compounds and condensed tannins in pecan shells was found. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) show association between cultivars and the observed variables. The nutritional profile in the different cultivars showed the trends described in other countries, but this work shows some significant differences that could be attributed to the specific edaphoclimatic conditions of cultivation in Uruguay. Copyright © 2022 Ferrari, Gil, Heinzen, Zoppolo and Ibáñez. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). 653 $aAntioxidant activity (AA) 653 $aCarya illinoinensis 653 $aFatty acids 653 $aNuts 653 $aORAC 653 $aPLATAFORMA AGROALIMENTOS 653 $aPolyphenols 653 $aTannins 653 $aTocopherols 700 1 $aGIL, G. 700 1 $aHEINZEN, H. 700 1 $aZOPPOLO, R. 700 1 $aIBÁÑEZ, F. 773 $tFrontiers in Nutrition, 2022, volume 9, e868054. OPEN ACESS. doi: https://doi.org/10.3389/fnut.2022.868054
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|